NKG2D公司
Janus激酶3
白细胞介素12
生物
NK-92
转导(生物物理学)
白细胞介素21
细胞生物学
白细胞介素15
人口
CD49b
癌症免疫疗法
病毒载体
免疫疗法
K562细胞
细胞
淋巴因子激活杀伤细胞
免疫学
细胞毒性T细胞
免疫系统
T细胞
基因
白细胞介素
细胞因子
医学
遗传学
体外
环境卫生
生物化学
重组DNA
作者
Maria A. Streltsova,Eugene V. Barsov,Sofia A. Erokhina,Elena I. Kovalenko
标识
DOI:10.1016/j.jim.2017.08.003
摘要
Natural killer (NK) cells are capable of rapidly recognizing and efficiently killing tumor cells. This makes them a potentially promising agent for cancer immunotherapy. Additional genetic modifications of NK cells may further improve their anti-tumor efficacy. Numerous technical challenges associated with gene delivery into NK cells have significantly tempered this approach. We achieved efficient retroviral vector transduction of primary human NK cells that were stimulated by a combination of IL-2 and engineered K562 cells expressing membrane-bound IL-21. The activated NK cells were in less differentiated state and expressed NK cell activation receptors NKG2D, NKp30, CD16, and were highly HLA-DR-positive. This NK cell population was highly susceptible to the transduction by both GFP- and NGFR-expressing retroviral vectors, with transduction efficiency exceeding 50%. More mature CD57+ NK cell population was generally resistant to retroviral vector transduction because of poor response to the stimulation. Our findings may facilitate retroviral vector-mediated genetic engineering of human primary NK cells for future immunotherapies.
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