Prokaryotic Expression of Channel Catfish Herpesvirus ORF6 and ORF10 and Antibodies Preparation

Primers were designed according to the channel catfish virus open reading frame 6 and 10,and both fragments of 417 bp and 456 bp were produced by PCR.The recombinant expressing plasmid pET-32a-ORF6 and pET-32a-ORF10 were constructed and subsequently expressed in E.coli after induced with IPTG.The purified proteins were used to immune rabbits separately and antibodies were obtained.Western blot analysis indicated that the polyclonal antibodies of ORF10 could react specifically with the purified CCV virions demonstrating that the ORF10 is a structural protein of CCV.