先天免疫系统
免疫学
免疫系统
发病机制
先天性淋巴细胞
获得性免疫系统
免疫
外周血单个核细胞
浆细胞样树突状细胞
T细胞
生物
特应性皮炎
树突状细胞
流式细胞术
疾病
单核细胞
细胞
医学
病理
生物化学
体外
遗传学
作者
Seon‐Pil Jin,K. Lee,Yoon Ji Bang,Yun‐Hui Jeon,Sun-Young Jung,So‐Jung Choi,Ji Su Lee,Junhan Kim,Emma Guttman‐Yassky,Chung‐Gyu Park,Hyun Je Kim,Seung-Bum Hong,Dong Hun Lee
出处
期刊:Allergy
[Wiley]
日期:2024-05-31
卷期号:79 (6): 1584-1597
被引量:1
摘要
Abstract Background Efforts to profile atopic dermatitis (AD) tissues have intensified, yet comprehensive analysis of systemic immune landscapes in severe AD remains crucial. Methods Employing single‐cell RNA sequencing, we analyzed over 300,000 peripheral blood mononuclear cells from 12 severe AD patients (Eczema area and severity index (EASI) > 21) and six healthy controls. Results Results revealed significant immune cell shifts in AD patients, including increased Th2 cell abundance, reduced NK cell clusters with compromised cytotoxicity, and correlated Type 2 innate lymphoid cell proportions with disease severity. Moreover, unique monocyte clusters reflecting activated innate immunity emerged in very severe AD (EASI > 30). While overall dendritic cells (DCs) counts decreased, a distinct Th2‐priming subset termed “Th2_DC” correlated strongly with disease severity, validated across skin tissue data, and flow cytometry with additional independent severe AD samples. Beyond the recognized role of Th2 adaptive immunity, our findings highlight significant innate immune cell alterations in severe AD, implicating their roles in disease pathogenesis and therapeutic potentials. Conclusion Apart from the widely recognized role of Th2 adaptive immunity in AD pathogenesis, alterations in innate immune cells and impaired cytotoxic cells have also been observed in severe AD. The impact of these alterations on disease pathogenesis and the effectiveness of potential therapeutic targets requires further investigation.
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