适体
啶虫脒
三螺旋
化学
生物物理学
纳米技术
分子生物学
生物
材料科学
立体化学
农学
杀虫剂
益达胺
作者
Minxin Mao,Xiujin Chen,Yina Cai,Hanjie Yang,Cunzheng Zhang,Yi Zhang,Zhouping Wang,Chifang Peng
标识
DOI:10.1016/j.snb.2022.133148
摘要
The lateral flow assay (LFA) has received extensive attention and been widely used in various targets. However, the development of aptamer based LFA (Apt-LFA) is still far behind, especially for small molecule targets detection. The shortcoming of long response time severely hindered the Apt-LFA strip application in on-site detection of small molecules contaminants in environment and foods. Herein, we introduce bivalent triple helix aptamer (BTHA) and nanozyme yielding an accelerated and signal amplified LFA for acetamiprid detection. We obtained a BTHA with two 10 nt arms, which could shorten the response time between the aptamer and acetamiprid by 6 times, thus dramatically accelerating the detection of acetamiprid. Moreover, we integrate [email protected] nanozyme to further amplify the detection signal by catalyzing the color development of substrate. In conjunction with smartphone-based analytical device, the Apt-LFA strip enabled the on-site quantitative detection of acetamiprid residue. It is believed that our strategy will facilitate the development of Apt-LFA against small molecule contaminants.
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