循环肿瘤细胞
流式细胞术
细胞外小泡
液体活检
细胞仪
计算机科学
病理
医学
癌症
生物
免疫学
内科学
细胞生物学
转移
作者
A. Muchlińska,Julia Smentoch,Anna J. Żaczek,Natalia Bednarz-Knoll
出处
期刊:Cancers
[MDPI AG]
日期:2022-08-29
卷期号:14 (17): 4178-4178
标识
DOI:10.3390/cancers14174178
摘要
Tumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monitor cancer patients. Flow cytometry was considered as a method to detect CTCs; however, due to the lack of verification of the investigated cells’ identity, this method failed to reach clinical utility. Meanwhile, imaging flow cytometry combining the sensitivity and high throughput of flow cytometry and image-based detailed analysis through a high-resolution microscope might open a new avenue in CTC technologies and provide an open-platform system alternative to CellSearch®, which is still the only gold standard in this field. Hereby, we shortly review the studies on the usage of flow cytometry in CTC identification and present our own representative images of CTCs envisioned by imaging flow cytometry providing rationale that this novel technology might be a good tool for studying tumor dissemination, and, if combined with a high CTC yield enrichment method, could upgrade CTC-based diagnostics.
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