亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

A novel portable label-free electrochemical immunosensor for ultrasensitive detection of Aeromonas salmonicida in aquaculture seawater

杀鲑气单胞菌 检出限 海水 生物传感器 微分脉冲伏安法 色谱法 水产养殖 化学 材料科学 电化学 电极 纳米技术 循环伏安法 渔业 生物 物理化学 生态学
作者
Najib Ben Messaoud,Marília Barreiros dos Santos,Ana Vieira,Alejandro Garrido-Maestu,Begoña Espiña,Raquel B. Queirós
出处
期刊:Analytical and Bioanalytical Chemistry [Springer Science+Business Media]
卷期号:414 (22): 6591-6600
标识
DOI:10.1007/s00216-022-04219-9
摘要

Infectious diseases caused by Aeromonas salmonicida (A. salmonicida) have a huge impact and produce significant losses in aquaculture and fish farming. Fish pathogen early detection is a critical step for the rapid identification and prevention of these problems. This work presents a novel portable label-free ultrasensitive electrochemical immunosensor for A. salmonicida detection in seawater. It consists of a fluidic integrated electrochemical-cell-chip (ECC) with independent chambers enclosing three electrochemical cells (ECs). Anti-A. salmonicida (AbSalm) antibodies were covalently attached to the gold surface of the microfabricated electrodes and were used for the sensitive detection of A. salmonicida. The antibody-antigen immunoreaction was studied by enzyme-linked immunosorbent assay (ELISA), and the surface functionalization was characterized by using quartz crystal microbalance (QCM), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The performance of the developed immunosensor, in terms of sensitivity, repeatability, and specificity, was also studied. The linear working range varied between 1 and 107 CFU mL-1, with a limit of detection (LOD) as low as 1 CFU mL-1. The suitability of the immunosensor for real sample detection was successfully demonstrated via recovery studies performed in spiked seawater samples. The proposed technology supports the use of low-cost and portable instrumentation that concedes the ultrasensitive, simple, and fast quantification of the A. salmonicida. To the best of our knowledge, this is the first portable sensing system for the detection of A. salmonicida in seawater samples, which provides a promising online monitoring platform for the detection of this bacterium in aquaculture facilities.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
FashionBoy应助兴奋的嘉懿采纳,获得10
2秒前
6秒前
祖之微笑发布了新的文献求助10
12秒前
科研通AI2S应助科研通管家采纳,获得10
19秒前
37秒前
41秒前
量子星尘发布了新的文献求助10
42秒前
49秒前
1分钟前
1分钟前
1分钟前
祖之微笑发布了新的文献求助10
1分钟前
简因完成签到 ,获得积分10
1分钟前
英姑应助苹果果汁采纳,获得30
1分钟前
kaka完成签到,获得积分10
1分钟前
2分钟前
Jason发布了新的文献求助10
2分钟前
2分钟前
2分钟前
量子星尘发布了新的文献求助10
2分钟前
小乘号子发布了新的文献求助10
2分钟前
2分钟前
2分钟前
2分钟前
子平完成签到 ,获得积分0
3分钟前
科研通AI5应助哲别采纳,获得10
3分钟前
gszy1975完成签到,获得积分10
3分钟前
小乘号子发布了新的文献求助10
3分钟前
3分钟前
Akim应助小乘号子采纳,获得10
3分钟前
量子星尘发布了新的文献求助10
3分钟前
yi完成签到,获得积分10
3分钟前
英俊的铭应助执着的草丛采纳,获得10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
slayers完成签到 ,获得积分20
4分钟前
Billy完成签到,获得积分0
4分钟前
4分钟前
4分钟前
5分钟前
SYLH应助Billy采纳,获得10
5分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976665
求助须知:如何正确求助?哪些是违规求助? 3520756
关于积分的说明 11204771
捐赠科研通 3257528
什么是DOI,文献DOI怎么找? 1798733
邀请新用户注册赠送积分活动 877897
科研通“疑难数据库(出版商)”最低求助积分说明 806629