清脆的
Cas9
活体细胞成像
计算生物学
基因组编辑
DNA
回文
核酸
基因组
基因组DNA
生物
细胞
遗传学
基因
作者
Charlotte Van Tricht,Thierry Voet,Jeroen Lammertyn,Dragana Spasić
标识
DOI:10.1016/j.tibtech.2022.10.003
摘要
Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with RNA imaging is challenging. Consequently, due to its capacity to bind double-stranded DNA (dsDNA) and design flexibility, the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (CRISPR-Cas9) technology has sparked enormous interest over the past decade. In this review, we describe various nucleic acid (NA)- and protein-based (amplified) signal generation methods that achieve imaging of repetitive and single-copy sequences, and even single-nucleotide variants (SNVs), next to highly multiplexed as well as dynamic imaging in live cells. With future progress in the field, the CRISPR-(d)Cas9-based technology promises to break through as a next-generation cell-imaging technique.
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