Lumi-HOF@Tb as Probes for Multiple Ratiometric Fluorescence and Chemiluminescence Sensing of α-Glucosidase

化学发光 化学 鲁米诺 荧光 发光 光化学 葡萄糖氧化酶 猝灭(荧光) 生物传感器 水解 基质(水族馆) 组合化学 色谱法 生物化学 光电子学 材料科学 地质学 物理 海洋学 量子力学
作者
Haiyan Li,Shang-Qing Zhang,Mingli Chen,Jianhua Wang
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:94 (44): 15448-15455 被引量:54
标识
DOI:10.1021/acs.analchem.2c03487
摘要

The innovative assembly of luminescent hydrogen-bonded organic frameworks (HOFs) into multifunctional optical sensors is of great significance for developing advanced materials. Herein, we report a facile room-temperature synthesis strategy for the luminol HOF modified by Tb3+ (Lumi-HOF@Tb) and featuring sensitive chemiluminescence and fluorescence characteristics. Lumi-HOF@Tb is further pioneered as a dual-signal sensor for selective detection of α-glucosidase, a type of enzyme that plays a crucial role in the digestion of carbohydrates, and screening of its inhibitors. The sensor is constructed by combining the dual optical characteristics of luminol from the HOF and lanthanide ion assistance. From the hydrolysis of α-glucosidase and the 4-nitrophenyl-α-d-glucopyranoside (pNGP) substrate emerges the fluorescent luminol–p-nitrophenol (pNP) complex at 466 nm and changes the inner filter absorption to recover Tb3+ characteristic fluorescence at 546 nm; luminol also produces a chemiluminescence signal driven by H2O2 from additional glucose oxidase-catalyzed hydrolysis of α-d-glucose. Fluorescence and chemiluminescence assays for α-glucosidase activity have therefore been established and exhibit detection limits as low as 0.04 and 0.005 U L–1, respectively. This study not only presents the possibility of Ln3+-HOF-based sensors as intelligent optical materials by integration of fluorescence and chemiluminescence techniques but also demonstrates great potential for future applications in biosensing.
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