分子内力
荧光
合理设计
氢键
药品
肝损伤
光化学
药物设计
化学
医学
药理学
纳米技术
立体化学
材料科学
计算化学
分子
有机化学
物理
量子力学
作者
Xue Chen,Lei Shi,Xiaoyun Ran,Lina Zhang,Kunpeng Xie,Yu Zhao,Jie Chen,Ling Ye,Xiao‐Qi Yu,Kun Li
出处
期刊:ACS materials letters
[American Chemical Society]
日期:2024-02-22
卷期号:6 (3): 1059-1068
标识
DOI:10.1021/acsmaterialslett.4c00002
摘要
Drug toxicity and related drug-induced liver injury (DILI) have become major biosafety issues. Enzyme-activated fluorescent probes have been demonstrated as a powerful tool for the diagnosis of DILI; however, they suffer from diffusive signal dilution and interference with other organs, thus leading to misassessment of drug toxicity and inaccurate diagnosis. Alkaline phosphatase (ALP) is an important biomarker, and alterations in the enzyme level are tightly correlated to the severity of liver damage. Herein, an enzyme-activated intramolecular hydrogen bond (IMHB) enhanced probe (TPEG-P) was developed for ALP detection in cells and mice models of DILI. Differing from previously reported intermolecular charge transfer (ICT) or the excited-state intramolecular proton transfer (ESIPT) mechanisms, the TPEG-P enables precise recognition and imaging of ALP only through the activation of intramolecular hydrogen bonds and could in situ sensitively detect varying degrees of liver injury caused by drug toxicity. This IMHB strategy will advance the development of enzyme-activated probes and precise bioimaging in the future.
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