活体细胞成像
小泡
荧光
细胞质
纳米技术
光漂白后的荧光恢复
光漂白
分辨率(逻辑)
活细胞
荧光寿命成像显微镜
细胞
化学
生物物理学
材料科学
细胞生物学
膜
生物
物理
光学
计算机科学
生物化学
人工智能
作者
Peng Xu,Simei Zhong,Yaohong Ma,Xinxin Duan,Yunfei Wei,Meng Zhang,Xin Jing,Yuhui Zhang
出处
期刊:ACS applied nano materials
[American Chemical Society]
日期:2024-01-12
卷期号:7 (2): 2120-2131
标识
DOI:10.1021/acsanm.3c05423
摘要
Vesicle entrapment remains a major bottleneck for the application of fluorescent nanoprobes (FNPs) with excellent optical properties in the field of super-resolution imaging of subcellular structures in living cells. Here, we developed a cell-penetrating peptide, fR8, which enables efficient cytoplasmic delivery of 3.5 nm FNPs instead of being trapped in vesicles, with a delivery efficiency of up to 75.6%. Further, we have successfully combined fusion protein technology with FNPs-fR8 to achieve high specificity and stable labeling of FNPs to various subcellular structures within live cells. Utilizing the advantages of FNPs, such as high brightness and resistance to photobleaching, FNPs modified with fR8 and the HaloTag ligand can be used for long-term super-resolution imaging of subcellular structures in living cells, allowing us to study the dynamic interactions between them. This work provides a practical tool for applying FNPs to live-cell super-resolution imaging and expands the application of super-resolution imaging technology in live-cell studies.
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