粪肠球菌
屎肠球菌
生物膜
抗菌剂
微生物学
肠球菌
体外
生物
细菌
最小抑制浓度
化学
生物化学
抗生素
金黄色葡萄球菌
遗传学
作者
Yuan Wu,Huilong Li,Pingyun Wu,Ruolan Wu,Pengfei She
标识
DOI:10.1093/jambio/lxae001
摘要
Abstract Aim The purpose of this study is to assess the in vitro antimicrobial and anti-biofilm effects of the anti-protozoal agent tafenoquine (TAF) on Enterococcus and elucidate its underlying mode of action. Methods and results The present work investigated the susceptibility of TAF on 3 type strains and 11 clinical isolates of enterococci. The results indicated that TAF exhibited powerful antimicrobial activity against both of Enterococcus faecalis and Enterococcus faecium with minimum inhibitory and bactericidal concentrations ranging from 8 to 16 µg ml−1. Meanwhile, biofilm inhibition and eradication assays showed that TAF exhibited potent anti-biofilm activity against E. faecalis ATCC 29212 and E. faecium ATCC 19434. Ultra-microscopic observations revealed significant changes in bacterial morphology and structure caused by TAF, particularly for the disruption of plasma membrane. Mechanistic investigations also revealed that TAF altered both membrane permeability and potential while also impacting adenosine triphosphate production as well as reactive oxygen species generation. In addition, no detectable cytotoxicity of TAF on human cells was observed at concentrations near the minimal inhibitory concentration. Conclusions In summary, this study confirmed that TAF could effectively inhibit Enterococcus as well as its biofilm formation.
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