副溶血性弧菌
适体
检出限
线性范围
镧系元素
荧光
探测器
生物系统
色谱法
化学
计算机科学
生物
物理
光学
分子生物学
有机化学
电信
细菌
遗传学
离子
作者
Kuiyu Wang,Lanxiang Zeng,Liangke Gong,Yusheng Ouyang,Tao Yang,Tianying Sun,Hui Zeng
标识
DOI:10.1016/j.snb.2023.133881
摘要
In this study, a rapid visual analysis strategy for pathogens was constructed based on lanthanide metal–organic frameworks (LnMOFs) and FAM-aptamer. The dual fluorescence signal changes were realized by the competitive binding effect of the target and LnMOFs on the FAM-aptamer. The nitrocellulose membrane (NC membrane) is used as the signal output platform, and the G/R value in Red, Green, Blue (RGB) of negative and positive signals is collected to output the detection results. Using Vibrio parahaemolyticus in seafood as model target, the detection performance of dual fluorescence sensing strategy was verified. Under the optimal conditions, the G/R difference between the positive and negative signals and the concentration of Vibrio parahaemolyticus was linear in the range of 102-105 colony forming units (CFU)/mL, and the detection limit was 102 CFU/mL. The method is simple to operate and the detection time can be controlled within 20 min. It can be developed as a portable detection kit, providing a new technical means for the safety and quality control of food supply chain.
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