Evaluation of Zein Nanoparticles as Delivery Agents of SARS-CoV-2 Antigens

抗原 病毒学 2019年冠状病毒病(COVID-19) 严重急性呼吸综合征冠状病毒2型(SARS-CoV-2) 纳米颗粒 2019-20冠状病毒爆发 生物 纳米技术 医学 材料科学 免疫学 传染病(医学专业) 疾病 病理 爆发
作者
Verónica A. Márquez-Escobar,María José Alonso-Cerda,Sergio Rosales‐Mendoza,Lourdes Betancourt-Mendiola
出处
期刊:Vaccines [Multidisciplinary Digital Publishing Institute]
卷期号:13 (2): 139-139
标识
DOI:10.3390/vaccines13020139
摘要

Background/Objectives: Nanovaccines have significant potential to enhance immunization strategies by improving efficacy, safety, and cost-effectiveness. In particular, organic nanoparticles hold promise for the generation of low-cost nanovaccines obtained by environmentally friendly methods. In this study, the feasibility of using zein nanoparticles (NPs) as carriers for an antigenic peptide (p30) and the receptor binding domain (RBD) from SARS-CoV-2 spike protein was explored. Methods: A synthesis method for zein NPs was established by combining previously reported techniques, and the resulting NPs were characterized in terms of morphology, particle size, polydispersity index (PDI), surface charge, and colloidal stability using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Tween 20 was employed as a surfactant to enhance particle stability and prevent aggregation. Results: The zein NPs were deemed safe based on an in vitro cytotoxicity assay using Vero cells. Immunogenicity assessments demonstrated that zein NPs:p30 and zein NPs:RBD induced IgG responses in test mice, whose magnitude was comparable to those achieved with alum as an adjuvant. Conclusions: These findings support the use of zein NPs as promising vaccine delivery vehicles with adjuvant effects due to their ease and environmentally friendly synthesis, high stability, and low cost.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
勤恳的半邪完成签到,获得积分10
刚刚
灯与鬼发布了新的文献求助10
刚刚
3秒前
ZERO完成签到,获得积分10
6秒前
ssssxr完成签到,获得积分20
7秒前
Neko发布了新的文献求助10
8秒前
8秒前
酸奶辣条发布了新的文献求助10
8秒前
13秒前
CodeCraft应助博修采纳,获得10
16秒前
16秒前
xu完成签到 ,获得积分10
16秒前
领导范儿应助PXP采纳,获得10
18秒前
欧阳正义发布了新的文献求助10
21秒前
21秒前
深情安青应助夔kk采纳,获得30
22秒前
xzzt发布了新的文献求助10
22秒前
23秒前
24秒前
安白完成签到 ,获得积分20
26秒前
hoshi发布了新的文献求助10
26秒前
我陈雯雯实名上网完成签到,获得积分10
26秒前
27秒前
PXP发布了新的文献求助10
30秒前
31秒前
32秒前
33秒前
博修发布了新的文献求助10
35秒前
芒芒发布了新的文献求助200
36秒前
36秒前
烟花应助飞天817采纳,获得10
36秒前
36秒前
饱满的书萱完成签到,获得积分20
37秒前
40秒前
李健应助博修采纳,获得10
40秒前
41秒前
李健应助刘先生采纳,获得10
41秒前
42秒前
43秒前
wanci应助Shennnn采纳,获得10
43秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3967279
求助须知:如何正确求助?哪些是违规求助? 3512575
关于积分的说明 11164253
捐赠科研通 3247522
什么是DOI,文献DOI怎么找? 1793850
邀请新用户注册赠送积分活动 874729
科研通“疑难数据库(出版商)”最低求助积分说明 804495