上睑下垂
促炎细胞因子
间充质干细胞
半胱氨酸蛋白酶1
信使核糖核酸
乳酸脱氢酶
缺血
小胶质细胞
基因敲除
细胞生物学
化学
细胞凋亡
程序性细胞死亡
医学
生物
免疫学
炎症
内科学
生物化学
酶
基因
作者
Kuang Pan,Qingxia Peng,Zhen Huang,Zhaofei Dong,Wei-Jye Lin,Yidong Wang
标识
DOI:10.1016/j.jstrokecerebrovasdis.2023.107199
摘要
Pyroptosis is a new type of programmed cell death that has a strong proinflammatory effect. The present study investigated the dynamic changes of pyroptosis-related molecules and the effect of mesenchymal stem cells (MSCs) on pyroptosis following cerebral ischemia/reperfusion (I/R).The temporal pattern and cellular distribution of caspase-1, Gasdermin D and E (GSDMD and GSDME) in the peri-infarct area, and the effect of human MSCs on GSDMD, IL-1β, IL-18, Lactate dehydrogenase (LDH) and neurological function were studied in a rat model of transient focal cerebral ischemia.The expression of caspase-1 mRNA increased with time, with a protein level of pro-caspase-1 comparable to its mRNA level, while the level of cleaved-caspase-1 protein peaked at 48 h following I/R. Increased levels of GSDMD mRNA and protein were also observed, with a peak level at 24 h. There were no significant changes in GSDME mRNA or protein expression after I/R. In regards to changes in the number of cells expressing GSDMD after I/R, that for neurons was more significant than those for microglia and astrocytes. The modified neurological severity score discrepancy and the expression of GSDMD showed no significant differences within 24 h following I/R between the MSC- and NS-treated groups, but MSCs treatment promoted the secretion of IL-1β, IL-18 and LDH.In the early stage of cerebral infarction in rats, there were dynamic changes in pyroptosis-related molecules (caspase-1 and GSDMD), but MSCs showed no effect on the levels of GSDMD or neurological function.
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