叶状天疱疮
天疱疮
桥粒蛋白
寻常性天疱疮
桥粒芯糖蛋白1
转录组
生物
免疫学
B细胞
自身免疫性疾病
自身抗体
基因
基因表达
抗体
遗传学
作者
Shohei Egami,Takashi Watanabe,Ayano Fukushima‐Nomura,Hisashi Nomura,Hayato Takahashi,Jun Yamagami,Osamu Ohara,Masayuki Amagai
标识
DOI:10.1016/j.jid.2023.03.1661
摘要
Autoreactive B cells are assumed to play a critical role in pemphigus; however, the characteristics of these cells are not yet fully understood. In this study, 23 pemphigus vulgaris or pemphigus foliaceus samples were used to isolate circulating desmoglein (DSG)-specific B cells. Transcriptome analysis of the samples was performed at the single-cell level to detect genes involved in disease activity. DSG1- or DSG3-specific B cells from three patients' differentially expressed genes related to T cell costimulation (CD137L) as well as B-cell differentiation (CD9, BATF, TIMP1) and inflammation (S100A8, S100A9, CCR3), compared with nonspecific B cells from the same patients. When the DSG1-specific B cells before and after treatment transcriptomes of the patient with pemphigus foliaceus were compared, there were changes in several B-cell activation pathways not detected in non-DSG1-specific B cells. This study clarifies the transcriptomic profile of autoreactive B cells in patients with pemphigus and documents the gene expression related to disease activity. Our approach can be applied to other autoimmune diseases and has the potential for future detection of disease-specific autoimmune cells.
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