黄素组
脱落酸
黄素单核苷酸
生物
黄素腺嘌呤二核苷酸
突变体
拟南芥
生物化学
拟南芥
核黄素
转录因子
生物合成
转录组
基因表达
细胞生物学
基因
辅因子
酶
作者
Junya Namba,Masaki Harada,Rei Shibata,Yuki Toda,Takanori Maruta,Takahiro Ishikawa,Shigeru Shigeoka,Kazuya Yoshimura,Takahisa Ogawa
出处
期刊:Plant Science
[Elsevier]
日期:2024-10-01
卷期号:347: 112196-112196
标识
DOI:10.1016/j.plantsci.2024.112196
摘要
Riboflavin (RF) serves as a precursor to flavin mononucleotide and flavin adenine dinucleotide, which are crucial cofactors in various metabolic processes. Strict regulation of cellular flavin homeostasis is imperative, yet information regarding the factors governing this regulation remains largely elusive. In this study, we first examined the impact of external flavin treatment on the Arabidopsis transcriptome to identify novel regulators of cellular flavin levels. Our analysis revealed alterations in the expression of 49 putative transcription factors. Subsequent reverse genetic screening highlighted a member of the dehydration-responsive element binding (DREB) family, AtDREB2G, as a potential regulator of cellular flavin levels. Knockout mutants of AtDREB2G (dreb2g) exhibited reduced flavin levels and decreased expression of RF biosynthetic genes compared to wild-type plants. Conversely, conditional overexpression of AtDREB2G led to an increase in the expression of RF biosynthetic genes and elevated flavin levels. In wild-type plants, exposure to low temperatures and abscisic acid treatment stimulated enhanced flavin levels and upregulated the expression of RF biosynthetic genes, concomitant with the induction of AtDREB2G. Notably, these responses were significantly attenuated in dreb2g mutants. Our findings establish AtDREB2G is involved in the positive regulation of flavin biosynthesis in Arabidopsis, particularly under conditions of low temperature and abscisic acid treatment.
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