Evaluation of the antibacterial activity of antibiotics with different antibacterial mechanisms using electrochemical signals of heat‐treated Escherichia coli

Electrochemistry with purines as indicators may be a promising new method for antibiotic activity testing. However, the traditional electrochemical detection method (S‐LSV) requires a long accumulation time for purines and has low signal intensity. High‐temperature treatment of bacterial samples may significantly enhance the signal intensity and speed of detection. However, this process may also impact the structure and metabolism of bacteria. Further research is necessary to determine how electrochemical signals respond to antibiotic antibacterial activity. In this paper, the electrochemical detection method based on heat‐treated Escherichia coli ( <i>E. coli</i>, H‐LSV) was used to investigate the