Insect Resistance in Quercus robur: A Comparative Metabolomic and Transcriptomic Analysis of Normal and Curly Leaf Varieties

Quercus robur is a tree species that produces acorns with rich nutritional value. Its leaf rolling is crucial for adapting to stress, but this trait's impact on plant-insect interactions remains unknown. We compared the resistance phenotypes, metabolomic profiles, and transcriptomic data of the curly leaf (Qr-T) and normal-leaf (Qr-S) varieties of Q. robur. Findings revealed that Qr-T exhibited higher leaf area consumption under herbivory. Metabolomic analysis found lower levels of key defensive compounds like Questiomycin A, Caffeine, and Indoleacrylic acid in Qr-T. Transcriptomics revealed up-regulation of DEGs related to development (e.g., MYO17, LEC2) and down-regulation of defense-related DEGs (like IOS1, Y3471) in Qr-T. GO and KEGG analyses indicated that defense responses and the isoflavonoid biosynthesis pathway were suppressed in Qr-T. Coexpression network analysis identified coordinated down-regulation of defense-related genes, such as CYP81Q32 and CYP94A5, and their coexpressed transcription factors (such as WRKY6, WRKY53) in Qr-T. The above findings suggest that leaf curling of Q. robur may weaken its insect resistance, which provides clues for improving plant resistance in agroforestry.