生物分子
量子点
电化学发光
膜
纳米技术
生物传感器
葡萄糖氧化酶
材料科学
化学
检出限
色谱法
生物化学
作者
Lei Wang,Zhi‐Gang Wang,Di Ning,Yusi Hu,Shu‐Lin Liu,Dai‐Wen Pang
出处
期刊:Nano Today
[Elsevier]
日期:2023-06-01
卷期号:50: 101855-101855
被引量:11
标识
DOI:10.1016/j.nantod.2023.101855
摘要
Electrochemiluminescence (ECL) offers numerous unique advantages for microscopic imaging. However, achieving real-time dynamic observation of biomolecules on cell membranes through ECL imaging remains a challenge. Herein, we present a new ECL nanoemitter for real-time multicolor imaging of biomolecules on cell membranes. The nanoemitter consists of quantum dots (QDs) with surface-immobilized glucose oxidase capable of catalyzing glucose oxidation to produce co-reactant hydrogen peroxide (H2O2). This nanoemitter enables sequential real-time imaging with a relatively high signal-to-noise ratio owing to the continuous availability of co-reactants. Therefore, by using the functionalized multicolor QDs for ECL-based single-particle tracking, the dynamic behaviors of individual biomolecules on cell membranes, including inter-molecular interactions, can be visualized in real time. This new nanoemitter is expected to facilitate investigations of the dynamics of biomolecules on cell membranes.
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