人参
MAPK/ERK通路
免疫系统
先天免疫系统
p38丝裂原活化蛋白激酶
刺激
化学
肿瘤坏死因子α
细胞因子
五加科
细胞生物学
药理学
信号转导
生物
生物化学
医学
免疫学
内分泌学
病理
替代医学
作者
Myoung‐Sook Shin,Ji Hoon Song,Pilju Choi,Jong Hun Lee,Song‐Yi Kim,Kwang‐Soon Shin,Jungyeob Ham,Ki Sung Kang
标识
DOI:10.1021/acs.jafc.8b00152
摘要
Panax ginseng Meyer has been used for the treatment of immune diseases and for strengthening the immune function. In this study, we evaluated the innate immune-stimulating functions and action mechanisms of white ginseng (WG) and heat-processed ginseng (HPG) in RAW264.7 cells. According to LC-MS analysis results, WG contained typical ginsenosides, such as Rb1, Rc, Rb2, Rd, and Rg1, whereas HPG contained Rg3, Rk1, and Rg5 as well as typical ginsenosides. HPG, not WG, enhanced NF-κB transcriptional activity, cytokine production (IL-6 and TNF-α), and MHC class I and II expression in RAW264.7 cells. In addition, HPG phosphorylated MAPKs and NF-kB pathways. In experiments with inhibitors, the ERK inhibitor completely suppressed the effect of HPG on IL-6 and TNF-α production. HPG-induced c-Jun activation was suppressed by an ERK inhibitor and partially suppressed by JNK, p38, and IκBα inhibitors. Collectively, these results suggested that HPG containing Rg3, Rg5, and Rk1 increased macrophage activation which was regulated by the ERK/c-Jun pathway in RAW264.7 cells.
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