HELP (Hydrogen peroxide electrochemical profiling): A novel biosensor for measuring hydrogen peroxide levels expressed in breath for monitoring airway inflammation using electrochemical methods

Breathomics is being explored as a non-invasive pathway for disease diagnosis. In this paper we describe the design and fabrication of an electrochemical transduction-based sensing scheme that can be used for the non-invasive diagnosis of respiratory inflammation by monitoring hydrogen peroxide (H 2 O 2 ) vapors in breath. AuNP-nafion@[BMIM]BF 4 modified electrode forms the backbone of the electrochemical sensor platform. This is the first report of an electrochemical sensor platform for assessing airway inflammation by monitoring H 2 O 2 levels in the lower parts-per billion (50 ppb) range. We engineered the RTIL interaction with the target analyte through computational and chemical modeling. The results obtained via computational and electrochemical modeling helped in strengthening our premise and serve as a proof-of-concept for the advancement of a breathomics-aided electrochemical detection approach. • Label-free electrochemical approach using AuNP-nafion@[BMIM]BF 4 as transducing element for detection of hydrogen peroxide. • Optimization of RTIL-electrode interface through computational and chemical modeling to maximize the interaction of target analyte • Linear dynamic range of 50 PPB-800 PPB, Limit of detection of 50 PPB with 95% confidence interval. • Display reproducible sensing by varying the concentrations and validate the surface regeneration. • Specificity study to demonstrate selective sensing response the AuNP-nafion@[BMIM]BF 4 modified electrode system.