衣壳
DNA
转染
核酸酶
DNA折纸
基因
自组装
基因传递
DNA纳米技术
化学
生物物理学
计算生物学
生物
纳米技术
生物化学
材料科学
作者
Meiwen Cao,Zijin Zhang,Xiaoyang Zhang,Yu Wang,Jingjing Wu,Zhihong Liu,Li Sun,Dong Wang,Tongtao Yue,Yuchun Han,Yingxiong Wang,Yilin Wang,Ming Wang
标识
DOI:10.1016/j.jcis.2022.01.181
摘要
Smart artificial viruses have been successfully developed by co-assembly of de novo designed peptides with DNA, which achieved stimuli-responsibility and efficient gene transfection in cancer cells. The peptides were designed to incorporate several functional segments, including a hydrophobic aromatic segment to drive self-assembly, two or more cysteines to regulate the assemblage shape and stabilize the assembled nanostructures via forming disulfide bonds, several lysines to facilitate co-assembly with DNA and binding to cell membranes, and an enzyme-cleavable segment to introduce cancer sensitivity. The rationally designed peptides self-assembled into stable nanospheres with a uniform diameter of < 10 nm, which worked as capsid-like subunits to further interact with DNA to produce hierarchical virus-mimicking structures by encapsulating DNA in the interior. Such artificial viruses can effectively protect DNA from nuclease digestion and achieve efficient genome release by enzyme-triggered structure disassembly, which ensured a high level of gene transfection in tumor cells. The system emulates very well the structural and functional properties of natural viruses from the aspects of capsid formation, genome package and gene transfection, which is highly promising for application as efficient gene vectors.
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