The relationship between the structure and functionality of essential PUFA delivery systems based on sodium caseinate with phosphatidylcholine liposomes without and with a plant antioxidant: an in vitro and in vivo study

磷脂酰胆碱 多不饱和脂肪酸 脂质体 鱼油 化学 超分子化学 水溶液 二十碳五烯酸 抗氧化剂 溶解度 色谱法 化学工程 有机化学 磷脂 分子 生物化学 脂肪酸 渔业 工程类 生物
作者
M.G. Semenova,Anna S. Antipova,E. I. Martirosova,Sergey Chebotarev,N. P. Palmina,Natalya G. Bogdanova,N. I. Krikunova,Daria Zelikina,Maria S. Anokhina,Valery V. Kasparov
出处
期刊:Food & Function [Royal Society of Chemistry]
卷期号:13 (4): 2354-2371 被引量:4
标识
DOI:10.1039/d1fo03336k
摘要

The aim of this work was to establish the main relationship between the structure and functionality of supramolecular complexes formed by sodium caseinate (SC) with phosphatidylcholine (PC) liposomes filled with fish oil (FO) to an equal mass ratio of n-3 to n-6 polyunsaturated fatty acids (PUFA) in the absence and presence of one of the most effective plant antioxidants, namely the essential oil of clove buds (EOC). The functionality of the supramolecular complexes (SC-PC-FO and SC-PC-FO-EOC) was considered from the point of view of the possibility of their use as effective delivery systems for long-chain n-3 PUFAs (eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids from FO). The laser light scattering method was used in the static, dynamic and electrophoretic modes to characterize the structure and thermodynamic parameters of the supramolecular complexes in an aqueous medium. It was found that the SC-PC-FO and SC-PC-FO-EOC complex particles had the following similar properties: nanosize; a spherical shape; 100% solubility in an aqueous medium (pH 7.0, ionic strength = 0.001 M); a high encapsulating ability of SC (up to 70%) in relation to the studied liposomes; and a high protective ability relative to lipid autooxidation (up to 96% on the 20th day of storage at room temperature in light). In addition, a sequential transformation of both the structural and thermodynamic parameters has been observed for the complex particles under in vitro simulated gastrointestinal (GI) conditions in accordance with the INFOGEST protocol. A greater release of the encapsulated lipids from the enzymatically hydrolyzed complex particles was observed at the small intestine stage compared to their release at the gastric stage. These data were in good agreement with those on the assessment of the bioavailability of the target PUFAs in in vivo experiments based on the chronic intake of aqueous solutions of the complexes (both SC-PC-FO and SC-PC-FO-EOC) by experimental mice for 92 days. Liver lipid profiles of the mice, obtained by gas-liquid chromatography, showed the following: (i) an almost twofold increase in the DHA content as compared with that of the control; (ii) an almost threefold decrease in the mass ratio of arachidonic acid (AA) (C20:4 n-6) to DHA (C22:6 n-3) compared to that of the control due to both a significant decrease in the AA content and a simultaneous pronounced increase in the DHA content; and (iii) an almost twofold decrease in the mass ratio of the total amounts of n-6 to n-3 PUFAs compared to that of the control.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
英勇皮卡丘完成签到,获得积分10
刚刚
Deny发布了新的文献求助10
刚刚
刚刚
刚刚
刚刚
xinanan完成签到,获得积分10
刚刚
荔枝多酚完成签到,获得积分10
1秒前
Coraline发布了新的文献求助10
1秒前
坦率的匪举报金闪闪求助涉嫌违规
1秒前
GGGGGG果果发布了新的文献求助10
1秒前
1秒前
2秒前
2秒前
64658应助Ruby采纳,获得10
2秒前
kiki完成签到 ,获得积分10
3秒前
小二郎应助fafamimireredo采纳,获得10
3秒前
4秒前
小胖熊完成签到,获得积分10
4秒前
4秒前
bgt发布了新的文献求助10
5秒前
张灬小胖完成签到,获得积分10
5秒前
Mmm发布了新的文献求助10
5秒前
星辰大海应助hhh采纳,获得10
5秒前
5秒前
5秒前
6秒前
6秒前
6秒前
joni完成签到,获得积分10
6秒前
111完成签到,获得积分10
7秒前
会走路的番茄完成签到,获得积分10
7秒前
汉堡包应助闪闪的梦柏采纳,获得10
7秒前
可爱的函函应助菠菜采纳,获得200
7秒前
8秒前
Jenny_Zhan完成签到,获得积分10
8秒前
9秒前
JoshuaChen发布了新的文献求助10
9秒前
火星上香菇完成签到,获得积分10
10秒前
10秒前
暮歌发布了新的文献求助50
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Handbook of Marine Craft Hydrodynamics and Motion Control, 2nd Edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3987078
求助须知:如何正确求助?哪些是违规求助? 3529488
关于积分的说明 11245360
捐赠科研通 3267987
什么是DOI,文献DOI怎么找? 1804013
邀请新用户注册赠送积分活动 881270
科研通“疑难数据库(出版商)”最低求助积分说明 808650