化学
酶
计算生物学
分子生物学
色谱法
生物
生物化学
作者
Mahdis Sadat Tabatabaei,Marya Ahmed
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 115-134
被引量:120
标识
DOI:10.1007/978-1-0716-2376-3_10
摘要
Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA assay, and clearly outline protocols for different types of ELISA assays, including direct, indirect, sandwich, competitive, and nanoparticle-based ELISA.
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