大肠杆菌
生物合成
尿苷
尿苷二磷酸葡萄糖
生物化学
代谢工程
基因
化学
尿苷二磷酸
发酵
转移RNA
尿苷三磷酸
酶
生物
核糖核酸
核苷酸
作者
Zeyu Li,Yingying Zhu,Pan Zhang,Wenli Zhang,Wanmeng Mu
标识
DOI:10.1021/acs.jafc.2c02426
摘要
Recently, human milk oligosaccharides (HMOs) have attracted increasing attention and display great commercial importance, especially for the infant formula industry. Lacto-N-tetraose (LNT) is an important neutral HMO commercially added in infant formula and a core structure for synthesizing complex HMOs. Previously, a novel LNT-generating β-1,3-galactosyltransferase from Pseudogulbenkiania ferrooxidans was identified and used for construction of an LNT-producing engineered Escherichia coli. In this work, LNT biosynthesis was further enhanced by pathway optimization and uridine 5'-triphosphate (UTP) regeneration. The main strategies included genomic integration of UDP-glucose 4-epimerase-encoding gene, fine-tuning of the LNT pathway-related genes, blocking of competitive pathways related to UDP-galactose, and overexpression of UTP supply related genes. The maximal LNT titer reached 6.16 and 57.5 g/L by shake-flask and fed-batch fermentation, respectively.
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