Sustainable upgrading of biological municipal wastewater treatment based on anammox: From microbial understanding to engineering application

Anaerobic ammonium oxidation (anammox) has drawn increasing attention as a promising option to energy-neutral wastewater treatment. While anammox process still faces challenges in the low-strength and organics-contained municipal wastewater due to its susceptibility and the technical gaps in substrate supply. Effective strategies for extensive implementation of anammox in municipal wastewater treatment plants (WWTPs) remain poorly summarized. In view of the significance and necessity of introducing anammox into mainstream treatment, the growing understanding not only at level of microbial interactions but also on view of upgrading municipal WWTPs with anammox-based processes need to be considered urgently. In this review, the critical view and comprehensive analysis were offered from the perspective of microbial interactions within partial nitrification- and partial denitrification-based anammox processes. To minimize the microbial competition and enhance the cooperation among anammox bacteria and other functional bacteria, targeted control strategies were systematically evaluated. Based on the comprehensive overview of recent advances, the combination of flexible regulation of input organic carbon with anaerobic/oxic/anoxic process and the integration of sludge fermentation with anoxic biofilms in anaerobic/anoxic/oxic process were proposed as promising solutions to upgrade municipal WWTPs with anammox technology. Furthermore, a new perspective of coupling anammox with denitrifying dephosphatation was proposed as a promising method for in-depth nutrients removal from carbon-limit municipal wastewater in this study. This review provides the critical and comprehensive viewpoints on anammox engineering in municipal wastewater and paves the way for the anammox-based upgrading of municipal WWTPs.