SIMULTANEOUS EXTRACTION AND SEPARATION OF MARTRINE, SOPHORIDINE AND SOPHOCARPINE FROM SOPHORA FLAVESCENS AIT BY RP-HPLC WITH ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY

The simultaneous extraction and separation of martrine, sophoridine and sophocarpine from Sophora flavescens Ait by RP-HPLC was developed by liquid-liquid extraction. The optimum extraction conditions were established using different extraction solvents, procedures and time. The extracts were separated and determined using a C18 column with methanol/water/diethylamine (55/45/0.10, v/v) as the mobile phase. Different preparative columns with different packing sizes were used to isolate the three compounds from the extracts, and the column with a 12 μm particle size showed better separation. The amounts and recovery of sophocarpine, sophoridine and martrine obtained were 0.081 mg/g, 0.031 mg/g and 0.051 mg/g, and 85.38%, 87.14% and 76.5%, respectively.