Relationship between Nuclear Remodeling and Development in Nuclear Transplant Rabbit Embryos1

生物 原核 卵裂球 胚泡 男科 细胞生物学 胚胎 卵母细胞 细胞质 合子 胚胎发生 医学
作者
Philippe Collas,James M. Robl
出处
期刊:Biology of Reproduction [Oxford University Press]
卷期号:45 (3): 455-465 被引量:154
标识
DOI:10.1095/biolreprod45.3.455
摘要

The present study characterized the profile of nuclear remodeling in nuclear transplant rabbit embryos and investigated the relationship between chromatin behavior after transfer and embryo development. The developmental potential and pattern of remodeling of donor nuclei from cleavage-, morula-, and blastocyst- (inner cell mass ICM, and trophectoderm, TE) stage donors were evaluated. In addition, we determined whether a modification in the synchrony between blastomere fusion and oocyte activation altered the profile of nuclear remodeling and affected development of reconstituted embryos. Development to blastocysts was similar with 8- and 32-cell-stage donor nuclei (42% and 33%, respectively, p > 0.1). However, it was reduced with ICM transplants (17%, p < 0.05), and development of TE transplants did not progress beyond the 8-cell stage. Upon blastomere fusion into nonactivated oocyte cytoplasm, nuclear remodeling was characterized by premature chromosome condensation (PCC), followed by pronuclear (PN) formation and swelling. PCC occurred synchronously within 1.2–1.5 h post-fusion with all stages of donor nuclei (p > 0.1). PN formation in 8- and 32-cell transplants occurred approximately 4 h after fusion, and was synchronous to that of female pronuclei in activated oocytes; however, it was delayed in ICM and TE transplants (p < 0.01). With all stages of donor nuclei, final nuclear diameter was similar to, or larger than, that of female pronuclei. Fusion to activated oocyte cytoplasm, as opposed to nonactivated cytoplasm, prevented PCC and extensive nuclear swelling (16.0 ± 0.7 vs. 30 ± 0.7 µm, respectively, p < 0.01). Nuclear diameter in early embryos was smaller (p < 0.01), and development to blastocysts was reduced (p < 0.05). The results indicate that remodeling of the donor nucleus is not essential for development to blastocysts; however, it is beneficial. Furthermore, complete reprogramming seems possible only after remodeling of the donor nucleus, i.e., PCC in nonactivated cytoplasm, followed by nuclear swelling upon activation of the oocyte.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
3秒前
帅气的藏鸟完成签到,获得积分10
7秒前
加油完成签到 ,获得积分10
8秒前
健康的宛菡完成签到 ,获得积分10
9秒前
橙果果发布了新的文献求助20
9秒前
晚晚完成签到,获得积分10
10秒前
10秒前
听闻韬声依旧完成签到 ,获得积分10
10秒前
ZHZ完成签到,获得积分10
11秒前
啊哈啊哈额完成签到,获得积分10
11秒前
yyy完成签到,获得积分10
12秒前
12秒前
xiaoputaor完成签到 ,获得积分10
13秒前
Camus发布了新的文献求助10
13秒前
paper reader完成签到,获得积分10
13秒前
14秒前
八八九九九1完成签到,获得积分10
15秒前
tigger完成签到 ,获得积分10
16秒前
18秒前
20秒前
优雅的千雁完成签到,获得积分10
22秒前
2316690509完成签到 ,获得积分10
22秒前
没用的三轮完成签到,获得积分10
22秒前
fancy完成签到 ,获得积分10
22秒前
mayberichard完成签到,获得积分10
26秒前
LINDENG2004完成签到 ,获得积分10
32秒前
wz完成签到,获得积分10
33秒前
简奥斯汀完成签到 ,获得积分10
40秒前
五本笔记完成签到 ,获得积分10
40秒前
43秒前
花花发布了新的文献求助20
43秒前
asd113发布了新的文献求助10
47秒前
美满的小蘑菇完成签到 ,获得积分10
47秒前
自然白安完成签到 ,获得积分10
53秒前
量子星尘发布了新的文献求助10
56秒前
等待小鸽子完成签到 ,获得积分10
58秒前
龙虾发票完成签到,获得积分10
1分钟前
小康学弟完成签到 ,获得积分10
1分钟前
了0完成签到 ,获得积分10
1分钟前
慕青应助科研通管家采纳,获得10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038066
求助须知:如何正确求助?哪些是违规求助? 3575779
关于积分的说明 11373801
捐赠科研通 3305584
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022