Relationship between Nuclear Remodeling and Development in Nuclear Transplant Rabbit Embryos1

生物 原核 卵裂球 胚泡 男科 细胞生物学 胚胎 卵母细胞 细胞质 合子 胚胎发生 医学
作者
Philippe Collas,James M. Robl
出处
期刊:Biology of Reproduction [Oxford University Press]
卷期号:45 (3): 455-465 被引量:154
标识
DOI:10.1095/biolreprod45.3.455
摘要

The present study characterized the profile of nuclear remodeling in nuclear transplant rabbit embryos and investigated the relationship between chromatin behavior after transfer and embryo development. The developmental potential and pattern of remodeling of donor nuclei from cleavage-, morula-, and blastocyst- (inner cell mass ICM, and trophectoderm, TE) stage donors were evaluated. In addition, we determined whether a modification in the synchrony between blastomere fusion and oocyte activation altered the profile of nuclear remodeling and affected development of reconstituted embryos. Development to blastocysts was similar with 8- and 32-cell-stage donor nuclei (42% and 33%, respectively, p > 0.1). However, it was reduced with ICM transplants (17%, p < 0.05), and development of TE transplants did not progress beyond the 8-cell stage. Upon blastomere fusion into nonactivated oocyte cytoplasm, nuclear remodeling was characterized by premature chromosome condensation (PCC), followed by pronuclear (PN) formation and swelling. PCC occurred synchronously within 1.2–1.5 h post-fusion with all stages of donor nuclei (p > 0.1). PN formation in 8- and 32-cell transplants occurred approximately 4 h after fusion, and was synchronous to that of female pronuclei in activated oocytes; however, it was delayed in ICM and TE transplants (p < 0.01). With all stages of donor nuclei, final nuclear diameter was similar to, or larger than, that of female pronuclei. Fusion to activated oocyte cytoplasm, as opposed to nonactivated cytoplasm, prevented PCC and extensive nuclear swelling (16.0 ± 0.7 vs. 30 ± 0.7 µm, respectively, p < 0.01). Nuclear diameter in early embryos was smaller (p < 0.01), and development to blastocysts was reduced (p < 0.05). The results indicate that remodeling of the donor nucleus is not essential for development to blastocysts; however, it is beneficial. Furthermore, complete reprogramming seems possible only after remodeling of the donor nucleus, i.e., PCC in nonactivated cytoplasm, followed by nuclear swelling upon activation of the oocyte.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
逝月完成签到,获得积分10
刚刚
1秒前
Lainey完成签到,获得积分10
1秒前
CUN完成签到,获得积分10
2秒前
zhouxy完成签到,获得积分20
2秒前
6秒前
onlyan发布了新的文献求助10
6秒前
独特冰安发布了新的文献求助10
6秒前
落后的凝梦完成签到 ,获得积分10
6秒前
7秒前
8秒前
独特冰安发布了新的文献求助10
11秒前
12秒前
独特元蝶完成签到,获得积分20
13秒前
yolo发布了新的文献求助10
13秒前
失眠无声发布了新的文献求助10
14秒前
独特元蝶发布了新的文献求助10
15秒前
lei029发布了新的文献求助10
16秒前
舒适静丹完成签到,获得积分10
17秒前
iris完成签到,获得积分10
17秒前
20秒前
21秒前
可爱的函函应助onlyan采纳,获得10
21秒前
量子星尘发布了新的文献求助10
24秒前
26秒前
赘婿应助Yy采纳,获得10
29秒前
30秒前
31秒前
34秒前
35秒前
苗苗发布了新的文献求助10
35秒前
dudu发布了新的文献求助30
36秒前
李爱国应助不吃西瓜采纳,获得10
37秒前
罗里完成签到 ,获得积分10
37秒前
YanDongXu发布了新的文献求助10
37秒前
39秒前
啦啦完成签到,获得积分20
39秒前
wangying完成签到,获得积分10
42秒前
HIT_C完成签到,获得积分20
43秒前
隐形曼青应助野山采纳,获得10
44秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975378
求助须知:如何正确求助?哪些是违规求助? 3519775
关于积分的说明 11199621
捐赠科研通 3256067
什么是DOI,文献DOI怎么找? 1798124
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305