数字聚合酶链反应
计算生物学
聚合酶链反应
纳米技术
计算机科学
生物
遗传学
材料科学
基因
作者
Lei Cao,Xingye Cui,Jie Hu,Zedong Li,Jane Ru Choi,Qingzhen Yang,Min Lin,Li Ying Hui,Feng Xu
标识
DOI:10.1016/j.bios.2016.09.082
摘要
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. In the past three decades, PCR has evolved from end-point PCR, through real-time PCR, to its current version, which is the absolute quantitive digital PCR (dPCR). In this review, we first discuss the principles of all key steps of dPCR, i.e., sample dispersion, amplification, and quantification, covering commercialized apparatuses and other devices still under lab development. We highlight the advantages and disadvantages of different technologies based on these steps, and discuss the emerging biomedical applications of dPCR. Finally, we provide a glimpse of the existing challenges and future perspectives for dPCR.
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