Mitochondria-targeted ratiometric fluorescent probe based on FRET for bisulfite

A new FRET-based ratiometric fluorescent probe (DSPT) for the detection of bisulfite was developed by the dansyl-piperazine-benzothiazolium conjugate platform, in which CC conjugated with benzothiazolium was recognition unit and benzothiazolium moiety was mitochondria-targeted unit. The CC double bond group would undergo Michael addition reaction with bisulfite, triggering significant fluorescence ratio increasing which was linear to the concentrations of bisulfite. The probe can be used in real sample including dry white wine and tap water. Besides, the test strips for bisulfite were prepared. Moreover, cell imaging results indicate that this probe is cell-membrane permeable and mitochondria-targetable, and can be applied to monitor both exogenous and endogenous bisulfite in ratiometric fluorescence imaging.