合成生物学
模块化设计
化学
可扩展性
模块化(生物学)
封装(网络)
纳米技术
化学生物学
计算机科学
计算生物学
程序设计语言
生物
计算机网络
生物化学
遗传学
材料科学
数据库
作者
Katarzyna P. Adamala,Daniel A. Martin-Alarcon,Katriona R. Guthrie-Honea,Edward S. Boyden
出处
期刊:Nature Chemistry
[Springer Nature]
日期:2016-11-14
卷期号:9 (5): 431-439
被引量:291
摘要
Genetic circuits and reaction cascades are of great importance for synthetic biology, biochemistry and bioengineering. An open question is how to maximize the modularity of their design to enable the integration of different reaction networks and to optimize their scalability and flexibility. One option is encapsulation within liposomes, which enables chemical reactions to proceed in well-isolated environments. Here we adapt liposome encapsulation to enable the modular, controlled compartmentalization of genetic circuits and cascades. We demonstrate that it is possible to engineer genetic circuit-containing synthetic minimal cells (synells) to contain multiple-part genetic cascades, and that these cascades can be controlled by external signals as well as inter-liposomal communication without crosstalk. We also show that liposomes that contain different cascades can be fused in a controlled way so that the products of incompatible reactions can be brought together. Synells thus enable a more modular creation of synthetic biology cascades, an essential step towards their ultimate programmability.
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