Cultivation of a pig parvovirus in various cell cultures.

The susceptibility of several established cell lines of pig (LLC-PK1 = pig kidney; MPK = minipig kidney; PK15 = pig kidney; ESK = embryonic swine kidney), bovine (EBTr = embryonic bovine trachea), monkey (MA-104 = fetal rhesus monkey kidney) and human (HEL-299 = embryonic human lung) origin to porcine parvovirus was studied. The primary pig kidney cell cultures (pPK) were included in the study as the reference cell system. From the results it appeared that the virus only replicated in cell lines originated from swine. In particular the MPK and ESK cell lines showed a susceptibility similar to that observed for pPK cell cultures. Intranuclear inclusions and plaques were also induced in these cell systems. It appeared therefore that MPK and ESK cell lines both possess all the requirements for use in pig parvovirus studies.