Separation,Purification and Identification of Two Vibrio Flagellin

The vibro flagellin of Vibro alginolyticus and Vibro harveyi was pxtracted and then detected by high-speed centrifugal,cycles of differential centrifugation,vertical slab gel electrophoresis,western blotting and electronic microscopy identification.The result shows that the experiment has already separated the purificated flagellin of Vibro harveyi and the original flagellin extracts of Vibro alginolyticus succesfully.The purification of Vibro harveyi has three clear protein lines by SDS-PAGE(relative molecular weight are 39.7,41.9and 42.7 KDa).Transmission electronic microscopy discovers that the protein assumes classical filamentous.This suggests that the flagellar filament of Vibro harveyi is made up of three subunit of flagellin whose molecular weight are 39.7,41.9 and 42.7 KDa respectively.The original flagellin extracts of Vibro alginolyticus have five to nine protein lines by SDS-PAGE,and the protein whose molecular weight are 36.8 KDa have remarkable immunogenicity.