A method of massively isolating PWN larvae and obtaining sterile larvae

There were differences in specific gravity and movement between larva and adult of pine wood nematode in carboxymethyl cellulose sodium salt(CMC) 0.3% solution.And based on that a new method was developed to isolate a large amount of pine wood nematode larvae.And pine wood nematode larvae could be sterilized when treated in hydrogen peroxide 3% at 4 ℃ for 60 min.