质量细胞仪
核糖核酸
流式细胞术
生物
分子生物学
计算生物学
邻近连接试验
细胞生物学
细胞仪
基因
生物化学
表型
受体
作者
Andreas P. Frei,Felice-Alessio Bava,Eli R. Zunder,Elena W.Y. Hsieh,Shih‐Yu Chen,Garry P. Nolan,Pier Federico Gherardini
出处
期刊:Nature Methods
[Springer Nature]
日期:2016-01-25
卷期号:13 (3): 269-275
被引量:302
摘要
PLAYR (proximity ligation assay for RNA) enables highly multiplexed transcript quantification in combination with protein marker detection in single cells using flow or mass cytometry. To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins. In primary cells, we quantified multiple transcripts, with the identity and functional state of each analyzed cell defined on the basis of the expression of a separate set of transcripts or proteins. By expanding high-throughput deep phenotyping of cells beyond protein epitopes to include RNA expression, PLAYR opens a new avenue for the characterization of cellular metabolism.
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