水泡性口炎病毒
生物
病毒学
干扰素
蛋白激酶R
牛痘
病毒复制
病毒
RNA沉默
核糖核酸
基因
蛋白激酶A
激酶
RNA干扰
细胞生物学
遗传学
丝裂原活化蛋白激酶激酶
重组DNA
作者
Irene Lostalé‐Seijo,José Martı́nez-Costas,Javier Benavente
出处
期刊:Virology
[Elsevier]
日期:2016-01-01
卷期号:487: 104-111
被引量:11
标识
DOI:10.1016/j.virol.2015.10.009
摘要
We have previously shown that the replication of avian reovirus (ARV) in chicken embryo fibroblasts (CEF) is more resistant to the antiviral action of interferon (IFN) than the replication of vesicular stomatitis virus (VSV) or vaccinia virus (VV). In this study we examined the capacity of these three viruses to induce the expression of IFN when infecting avian cells. Efficient expression of both type-α and type-β IFNs, as well as of the double-stranded RNA (dsRNA)-activated protein kinase (PKR), takes place in ARV-infected CEF, but not in cells infected with VSV or VV. PKR expression is not directly induced by ARV infection, but by the IFN secreted by ARV-infected cells. IFN induction in ARV-infected cells requires viral uncoating, but not viral gene expression, a situation similar to that reported for apoptosis induction by ARV-infected cells. However, our results demonstrate that IFN induction by ARV-infected CEF occurs by a caspase-independent mechanism.
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