Development and validation of RP-HPLC method for the estimation of bumetanide in tablet formulation

A remarkably simple, selective and precise method to determine bumetanide in tablet dosage form was developed and validated using Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The LC separations was achieved on a bondapak C18 column in the isocratic mode using Glacial acetic acid: Tetrahydrofuran: Water: Methanol (2:5:45:50 v/v/v/v), as a mobile phase at flow rate 1.0 mL/min with UV detection at 254 nm. The retention time for bumetanide was 11.3 min. The method was validated by determining its sensitivity, precision, accuracy as per ICH guidelines. The % recovery was found to be as is simple, rapid, precise and accurate and hence, can be applied for routine quality control analysis of this drug in tablet dosage form.