Study of Glycation and Advanced Glycation on a Humanized Monoclonal Antibody

In the presented work, we reviewed the protein glycation effect on recombinant monoclonal antibodies (MAbs), and demonstrated an in vitro glycation model on rhuMAb A was a successful tool to evaluate site-specific glycation. We also implemented this model to study MAb glycation in three aspects: the formation of glycation, the change of glycation adducts under thermal stress, and the control of glycation adducts stability by formulation compositions.