The purpose of this study was to determine if a statistically significant reduction in muscle activity (p<0.05) occurs when prolonged disclusion time (>0.4 sec/excursion) is shortened to <0.4 sec/excursion with the Immediate Complete Anterior Guidance Development (ICAGD) enameloplasty. Forty-five symptomatic, fully informed subjects (29 female, 16 male) had their right and left disclusion times recorded with T-Scan III, while simultaneously, the bilateral masseter and anterior temporalis muscle activity was recorded electromyographically with BioEMG III (n=180 muscles). This recording was done twice, once pretreatment and again posttreatment (same day) after undergoing the ICAGD enameloplasty on the same day without changing electrodes. The Student's paired t-test was utilized to detect any significant change in the muscle activity levels between the pre- and posttreatment lateral excursive muscle contractions. Highly significant reductions were found in all four muscles' activities after shortening the pretreatment prolonged disclusion time to less than 0.4 seconds (p<0.0014); after Bonferroni correction (p<0.006). When properly performed, such that the posttreatment disclusion time is <0.4 sec/excursion, the ICAGD enameloplasty predictably reduces excursive muscle activity levels in the bilateral anterior temporalis and masseter muscles. Excursive muscle hyperactivity can be a source of lactic acid accumulation, muscular ischemia, and chronic myalgic temporomandibular joint dysfunction (TMD) symptoms. The ICAGD enameloplasty significantly reduces excursive muscle contractions after completion of the first ICAGD treatment session.