淀粉
发酵
生物燃料
克鲁维酵母
乙醇燃料
水解
糖
生物过程
淀粉酶
化学
纤维素乙醇
制浆造纸工业
食品科学
还原糖
酵母
生物技术
纤维素
生物化学
化学工程
工程类
生物
酿酒酵母
酶
作者
Clive Chong,Ku Syahidah Ku Ismail,A. H. Ma’Radzi
出处
期刊:IOP conference series
[IOP Publishing]
日期:2021-05-01
卷期号:765 (1): 012004-012004
被引量:1
标识
DOI:10.1088/1755-1315/765/1/012004
摘要
Abstract Nowadays bioethanol is being used extensively in fuel production because of its technical feasibility, economically competitive, and environmentally friendly. It is expected that biofuel will contribute to 30% of the global energy demand by 2050. Therefore, it is very important to investigate any cost-effective bioprocessing that can improve the overall production yield. The study aims to produce bioethanol from cassava starch by Kluyveromyces marxianus at a laboratory scale and a 5L fermentation rig. A separate hydrolysis and fermentation (SHF) process of cassava starch was introduced due to high sugar content in starch, using a thermoanaerobe able to reduce the cooling time after hydrolysis. A combination of 0.35% v/w amylase and 0.20% v/w amyloglucosidase used in the hydrolysis of cassava starch produced 19.18 g/L of sugar. A 15 g/L of K. marxianus showed to be the best yeast concentration which could produce the highest bioethanol, 42.85 g/L. When the laboratory scale was scaled up to 5 L fermentation, the result was comparable at 42.33 g/L. The same SHF parameters in laboratory scale had been proven to be effective in a larger scale fermentation based on similar results obtained. Simulation using SuperPro software indicated that 50.13% of starch can be converted into ethanol.
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