Extraction of Droplets for Bioanalysis Based on Superhydrophobic Hole

Abstract Superhydrophobic surfaces, which have the excellent characteristics of self-cleaning, low-adhesion and non-wetting, have been widely used in the field of biochemical analysis by limiting sample loss. In this paper, a method to generate droplets of appropriate volume by superhydrophobic holes was proposed. First, the super-hydrophobic surface with super-hydrophobic holes was fabricated by laser etching and low surface energy modification. Then, the possible states of droplets were analyzed. In addition, the effect of droplets size and hole size on the states of droplets were studied comprehensively. This method of droplets generation based on superhydrophobic holes has great potential applications in step-by-step biological experiments.