GPX4
基因敲除
细胞凋亡
癌症研究
活力测定
癌细胞
程序性细胞死亡
细胞培养
癌症
生物
细胞
细胞生长
谷胱甘肽
谷胱甘肽过氧化物酶
分子生物学
生物化学
酶
遗传学
作者
Masakatsu Fukuda,Yudai Ogasawara,Hiroyasu Hayashi,Atsushi Okuyama,Junya Shiono,Kinya Inoue,Hideaki Sakashita
出处
期刊:Anticancer Research
[Anticancer Research USA Inc.]
日期:2021-04-01
卷期号:41 (4): 1785-1792
被引量:20
标识
DOI:10.21873/anticanres.14944
摘要
Background/Aim: This study aimed to elucidate the role of glutathione peroxidase 4 (GPX4) on the sterol regulatory element binding proteins (SREBPs)-proliferation pathway in oral cancer cells, and determine its protein expression in oral cancer tissues. Materials and Methods: Quantitative RT-PCR and immunoblot analysis were carried out. Cell viability assay, apoptosis detection assay, immunohistochemistry and GPX4 knockdown were performed. Results: The levels of both GPX4 mRNA and protein were highest in SAS cells. GPX4 knockdown in SAS cells, a human oral squamous cell carcinoma cell line, using GPX4 siRNA resulted in a reduction in cell number, which appeared to be due to non-apoptotic cell death such as ferroptosis. Furthermore, SREBP was clearly down-regulated by GPX4 knockdown in SAS cells. Immunopositivity for GPX4 was revealed on the membrane of human oral squamous cell carcinoma cells, and this was correlated with p53 immunoreactivity. Conclusion: GPX4 appears to play an important role in oral cancer proliferation.
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