MAPK/ERK通路
细胞生物学
先天免疫系统
巨噬细胞极化
细胞因子
化学
信号转导
生物
免疫系统
巨噬细胞
免疫学
生物化学
体外
作者
Nak‐Yun Sung,Miso Yang,Du-Sup Song,Eui‐Baek Byun,Jae Kyung Kim,Jong‐Heum Park,Beom‐Seok Song,Ju-Woon Lee,Sanghyun Park,Hyunjin Park,Myung‐Woo Byun,Eui‐Hong Byun,Jae‐Hun Kim
标识
DOI:10.1016/j.ejphar.2013.02.059
摘要
Numerous studies have shown various relationships between foods with a high nutritional value and a robust immune response, particularly studies that have focused on host protection and cytokine networks. This study aimed to clarify the role played by the procyanidin trimer C1 in innate and adaptive immunity. Procyanidin C1 did not exert cytotoxicity at concentrations ranging from 7.8 to 62.5 μg/ml in macrophage cells; therefore, concentration of 62.5 μg/ml was used as the maximum dose of procyanidin C1 throughout subsequent experiments. Procyanidin C1 enhanced inducible nitric oxide synthase-mediated nitric oxide production in a concentration-dependent manner. In addition, procyanidin C1 functionally induced macrophage activation by augmenting the expression of cell surface molecules (CD80, CD86, and MHC II) and proinflammatory cytokine production (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6) via activation of mitogen-activated protein kinase (MAPK), e.g., p38, ERK, and JNK and nuclear factor (NF)-κB signaling pathways. Interestingly, procyanidin C1 effectively polarized T helper type 1 (Th1) by secreting Th1-mediated cytokines (interferon-γ, IL-12p70, and IL-2) and inducing splenocyte proliferation, indicating that procyanidin C1 contributes to Th1 polarization of the immune response. Accordingly, these findings confirms that the procyanidin C1 induces macrophage activation via NF-κB and MAPK pathways, leading to Th1 polarization in murine splenocytes, which suggests that procyanidin C1 regulates innate and adaptive immunity by macrophage activation and Th1 polarization.
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