医学
结节病
热休克蛋白
免疫学
肺结核
细胞因子
休克(循环)
病理
内科学
生物化学
基因
化学
作者
Anna Dubaniewicz,Piotr Trzonkowski,Mirosława Dubaniewicz-Wybieralska,Andrzej Dubaniewicz,Mahavir Singh,Andrzej Myśliwski
出处
期刊:Respirology
[Wiley]
日期:2007-05-01
卷期号:12 (3): 346-354
被引量:61
标识
DOI:10.1111/j.1440-1843.2007.01076.x
摘要
Background and objective: Sarcoidosis (SA) is a disorder of unknown aetiology. Mycobacterium tuberculosis heat shock proteins (Mtb‐hsp) have been considered as causative agents of SA. The role of Mtb‐hsp in the immune response in SA has not been investigated. Methods: Mtb‐hsp‐stimulated T‐cell subsets and Th1/Th2 cytokine patterns in the supernatant from peripheral blood mononuclear cell cultures from 22 SA patients, 20 tuberculosis (TB) patients and 20 healthy volunteers were compared using flow cytometry. Results: In unstimulated cultures, a significantly higher percentage of CD8 + αβ + T‐cells were present in SA versus controls. Similarly there was a significantly increased IL‐6 and decreased IL‐4 level in SA and significantly lower INF‐γ, IL‐2, IL‐4, IL‐10 production in TB versus controls. After Mtb‐hsp stimulation, there was a significantly increased TNF‐α, IL‐6, IL‐10 and decreased INF‐γ, IL‐2, IL‐4 production in SA and significantly increased TNF‐α, IL‐6 concentrations in TB versus controls. CD8 + γδ + IL‐4 + T‐cells were detected significantly less often in Mtb‐hsp‐induced cultures in SA versus controls. Comparing SA versus TB, CD4 + γδ + TCR‐cells were significantly increased in Mtb‐hsp‐induced cultures in TB versus controls and SA. Before stimulation, significantly increased IL‐6, IL‐10 and decreased IL‐4 level in SA versus TB was revealed, whereas Mtb‐hsp stimulation caused significantly increased IL‐10 and decreased IL‐4 concentrations in SA. Conclusions: After Mtb‐hsp stimulation, increased levels of pro‐inflammatory cytokines, TNF‐α and IL‐6 were found in sera from SA and TB patients in comparison with healthy controls; SA patients demonstrated the lowest levels of IL‐4 and the highest levels of IL‐10.
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