Extension of Survival in Bilaterally Adrenalectomized Mice by Implantation of SF-1/Ad4BP-Induced Steroidogenic Cells

内分泌学 内科学 类固醇生成因子1 肾包膜 皮质酮 脂肪组织 生物 肾上腺 间充质干细胞 激素 医学 细胞生物学 基因 转录因子 核受体 生物化学
作者
Tomoko Tanaka,Chikao Aoyagi,Kuniaki Mukai,Koshiro Nishimoto,Shohta Kodama,Toshihiko Yanase
出处
期刊:Endocrinology [The Endocrine Society]
被引量:4
标识
DOI:10.1210/endocr/bqaa007
摘要

Abstract Mesenchymal stroma/stem cells (MSCs) exist in adult tissues, such as adipose tissue and bone marrow, and differentiate into cells of multiple lineages. In previous studies, we found that MSCs differentiate into steroidogenic cells by forced expression of steroidogenic factor 1 (SF-1)/adrenal 4 binding protein (Ad4BP), the master regulator of steroidogenesis and differentiation of pituitary gonadotrophs, adrenal glands, and gonads. In this study, SF-1/Ad4BP-induced steroidogenic cells derived from mouse adipose tissue–derived MSCs (ADSCs) were implanted under the kidney capsule of bilateral adrenalectomized (bAdx) mice. bAdx mice did not survive after 7 days. However, 4 of 9 bAdx mice implanted with SF-1/Ad4BP-induced steroidogenic cells, 1 of 10 bAdx mice transplanted with control ADSCs, and bAdx mice transplanted with an adrenal gland survived for 30 days. Plasma corticosterone levels in bAdx mice implanted with SF-1/Ad4BP-induced steroidogenic cells and control ADSCs were 5.41 ± 2.26 ng/mL (mean ± SEM) and undetectable at 7 days after implantation, respectively. After removal of the kidney bearing the graft from the surviving mice at 30 days after implantation, plasma corticosterone was not detected in any of the samples. Immunohistochemical staining revealed SF-1/Ad4BP-positive cells under the capsule of the kidney. Although we performed an adrenocorticotropin (ACTH) loading test on bAdx mice implanted with SF-1/Ad4BP-induced steroidogenic cells, ACTH responsiveness was not observed. Implantation of steroidogenic cells derived from ADSCs into bAdx mice increased the basal plasma corticosterone level and extended the survival of bAdx mice, suggesting the capability of restoring steroidogenic cells by cell transplantation therapy for adrenal insufficiency.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Ava应助扎心采纳,获得10
2秒前
LELE完成签到 ,获得积分10
4秒前
FashionBoy应助anlikek采纳,获得10
4秒前
5秒前
ccx完成签到,获得积分10
7秒前
左右逢我完成签到 ,获得积分10
9秒前
123完成签到,获得积分10
10秒前
东皇太一完成签到,获得积分10
11秒前
小龚完成签到 ,获得积分10
13秒前
ZZ完成签到,获得积分10
15秒前
潇洒的小鸽子完成签到 ,获得积分10
16秒前
追梦小帅完成签到,获得积分10
16秒前
奋斗天德完成签到 ,获得积分10
16秒前
18秒前
杨文献完成签到,获得积分10
18秒前
20秒前
海人发布了新的文献求助20
21秒前
轩辕寄风完成签到,获得积分10
21秒前
小马甲应助anlikek采纳,获得10
22秒前
欢城完成签到,获得积分10
22秒前
23秒前
杨文献发布了新的文献求助10
25秒前
阿高完成签到 ,获得积分10
26秒前
superspace完成签到,获得积分10
28秒前
1257应助beikeyy采纳,获得10
29秒前
Amic完成签到 ,获得积分10
29秒前
anlikek完成签到,获得积分10
31秒前
于晏孙完成签到 ,获得积分10
31秒前
小居很哇塞完成签到,获得积分10
32秒前
张璋完成签到,获得积分10
36秒前
海人完成签到,获得积分10
37秒前
小蘑菇应助木光采纳,获得10
39秒前
41秒前
43秒前
43秒前
隐形荟完成签到 ,获得积分10
46秒前
稳重紫蓝完成签到 ,获得积分10
47秒前
kbg990818完成签到 ,获得积分10
47秒前
扎心发布了新的文献求助10
47秒前
49秒前
高分求助中
中国国际图书贸易总公司40周年纪念文集 大事记1949-1987 2000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
草地生态学 880
Threaded Harmony: A Sustainable Approach to Fashion 799
Basic Modern Theory of Linear Complex Analytic 𝑞-Difference Equations 550
中国有机(类)肥料 500
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3059778
求助须知:如何正确求助?哪些是违规求助? 2715655
关于积分的说明 7445758
捐赠科研通 2361283
什么是DOI,文献DOI怎么找? 1251388
科研通“疑难数据库(出版商)”最低求助积分说明 607751
版权声明 596467