A High-Performance Liquid Chromatographic Method for the Determination of Meropenem in Serum

色谱法 化学 校准曲线 检出限 分析物 磷酸 相关系数 蛋白质沉淀 美罗培南 醋酸 色谱检测器 甲醇 高效液相色谱法 分析化学(期刊) 生物化学 抗生素耐药性 统计 有机化学 数学 抗生素
作者
Demet Dinçel,Olcay Sağırlı,Gülaçtı Topcu
出处
期刊:Journal of Chromatographic Science [Oxford University Press]
卷期号:58 (2): 144-150 被引量:4
标识
DOI:10.1093/chromsci/bmz087
摘要

Abstract In this study, a new, sensitive and selective high-performance liquid chromatographic method was developed for the determination of meropenem (MEM) in human serum. In the developed method, C18 column (3.9 × 150 mm, 5 μm) was selected as stationary phase at 30°C, and methanol: acetic acid solution mixture was used as mobile phase with gradient program. Chromatographic separation was carried out at a flow rate of 1 mL/min, and detection was performed at 300 nm with diode array detector. Doripenem was selected as an internal standard, and the analytes were extracted from serum using protein precipitation method with ortho-phosphoric acid: methanol. Detection wavelength was selected as 300 nm. The developed method was validated according to International Council for Harmonisation (ICH) guidelines. The calibration curve was linear over a concentration range of 4–240 μg/mL with correlation coefficient of 0.9985. The limit of detection and limit of quantification values were found as 0.057 and 0.192 μg/mL, respectively. The validated method was successfully applied for the determination of MEM in human serum samples collected from patient volunteers at different time intervals, and therapeutic drug monitoring of MEM has been investigated.
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