噬菌体展示
融合蛋白
基因
基因组
噬菌体
分子生物学
质粒
噬菌体
寡核苷酸
生物
计算生物学
遗传学
重组DNA
抗体
大肠杆菌
作者
GEORGEP. SMITH,Jamie K. Scott
出处
期刊:Elsevier eBooks
[Elsevier]
日期:1995-01-01
卷期号:: 333-362
被引量:23
标识
DOI:10.1016/b978-0-12-765561-1.50028-x
摘要
A “fusion phage” is a filamentous virion, which displays a foreign peptide fused to a coat protein and harbors the gene for the fusion protein within its genome. This chapter discusses the basic method of fusion-phage technology and describes an application for which these surface expression vectors are particularly well suited—the construction of epitope libraries. In such a library, the phages display “random” foreign peptides encoded by degenerate synthetic oligonucleotides spliced into the coat protein gene, the library as a whole representing up to billions of peptide sequences. If a phage displays a peptide that is a strong ligand for an antibody or other binding protein, it can be readily purified out of a library, even one containing a vast excess of nonbinding clones. This chapter further describes complex libraries, in which the displayed ligands are whole-folded domains. A recombinant coat protein gene can be transplanted from the phage genome into a type of plasmid called a phagemid. Phagemids contain the filamentous phage intergenic region comprising the origins of plus- and minus-strand synthesis and all other cis-acting elements needed for the synthesis of ssDNA and packaging it into virions.
科研通智能强力驱动
Strongly Powered by AbleSci AI