两种聚（左旋乳酸-己内酯）静电纺纳米纤维膜的性能比较

BACKGROUND: Copolymerization or mixture of poly-L-lactide and polycaprolactone can improve the own properties of poly-L-lactide and polycaprolactone. However, the different ratio of each material, as well as the concentration of electrospun solution will result difference in performance. OBJECTIVE: To compare the performance between two kinds of P(LLA-CL)(75/25;50/50) electrospun nanofibers membranes, further more, to select the optimal option of raw material and relevant concentration of electrospun solution. DESIGN, TIME AND SETTING: The contrast observation experiment was performed at the Biological Material and Tissue Engineering Laboratory of Donghua University from September 2007 to November 2008. MATERIALS: Two kinds of raw material P(LLA-CL)(75/25;50/50) was added into spinning solvent with different quality ratios (4%, 6%, 8% or 10%) to prepare nanofibers membrances. METHODS: The morphous of nanofibers membrances was observed at the 10 kV accelerating voltage following coated interface. The mechanical properties of electrospun membranes were assayed by scanning electron microscopy (SEM). Water contact angle was detected by contact angle measurement. MTT method was used to determine cell adhesion and proliferation. MAIN OUTCOME MEASURES: Fiber morphology, hydrophilicity, mechanical property and biocompatibility of electrospun nanofiber membrance were observed. RESULTS: SEM results showed that the fiber morphology of P(LLA-CL)(50/50) 6% nanofibers membrance was better and more uniform diameter distribution. Mechanic test indicated nanofibers membrance of P(LLA-CL)(50/50) had longer breaking elongation but smaller breaking stress than nanofibers membrance of P(LLA-CL)(75/25). Hydrophilicity of P(LLA-CL)(75/25) fibers was better. MTT results proved that 6% and 8% P(LLA-CL)(50/50) nanofibers could enhance the adhesion and proliferation of endothelial cells on the surface of materials, and the cells had the best spreading and attachment on the nanofibers. CONCLUSION: Nanofibers membrane of 6% P(LLA-CL)(50/50) has full advantage of material properties.