内吞循环
内吞作用
细胞器
DNA
化学
生物物理学
小泡
费斯特共振能量转移
细胞生物学
生物
生物化学
细胞
荧光
膜
量子力学
物理
作者
Souvik Modi,Yamuna Krishnan
出处
期刊:Methods in molecular biology
日期:2011-01-01
卷期号:: 61-77
被引量:7
标识
DOI:10.1007/978-1-61779-142-0_5
摘要
A few cellular compartments maintain acidic environments in their interiors that are crucial for their proper function. Alteration in steady state organelle pH is closely linked to several diseases. Although a few probes exist to measure pH of cell compartments, each has several associated limitations. We present a high-performance pH sensor, a DNA nanoswitch, a convenient method to map spatiotemporal pH changes in endocytic pathways. DNA has been used to make a variety of nanoswitches in vitro . However, the present DNA nanoswitch functions as a pH sensing device equally efficiently intracellularly as it does in vitro. This DNA nanoswitch functions as a FRET-based pH sensor in the pH regime of 5.5–7, with high dynamic range between pH 5.8 and 7. It is efficiently engulfed by Drosophila hemocytes through endocytosis and can be used to measure the acidity of the endocytic vesicles that it marks during their maturation till their lysosomal stage.
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