蛋白激酶C
胞吐
生物
爪蟾
二酰甘油激酶
细胞生物学
皮质颗粒
基因亚型
颗粒(地质)
分泌物
激酶
生物化学
卵母细胞
基因
胚胎
古生物学
作者
Cameron B. Gundersen,Sirus A. Kohan,Qian Chen,Joseph Iagnemma,Joy A. Umbach
标识
DOI:10.1242/jcs.115.6.1313
摘要
Previous work has shown that phorbol esters or diacylglycerol trigger cortical granule exocytosis in Xenopus oocytes. We sought to identify the isoform(s) of protein kinase C (PKC) that mediate(s) this regulated secretory event. Because this process is initiated by lipid activators of PKC but is independent of calcium ions, we focused on the family of novel(calcium-independent) PKCs. Pharmacological investigations using Gö6976 and Gö6983 tended to exclude PKCδ, ϵ and μ as secretory triggers. Subcellular fractionation and immunoblot data revealed that these oocytes expressed all five members of the novel PKC family, but it was only PKCη that colocalized with cortical granules. Finally, expression of wild type or constitutively active forms of PKCδ and η strongly supported the conclusion that it is PKCη that initiates cortical granule exocytosis in these cells. These observations represent an important step in identifying the mechanism of secretory triggering in this system.
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